Agrobacterium tumefaciens-Mediated Gene Transfer in a Major Human Skin Commensal Fungus, Malassezia globosa

Although the fungal microbiome in human skin mainly comprises lipophilic yeasts, including Malassezia species, these microorganisms can cause various dermatitis conditions, pityriasis versicolor, seborrheic dermatitis, folliculitis, and atopic depending on host condition. Both globosa restricta are major species implicated Malassezia-related dermatitis. However, pathogenicity of has not been revealed at genetic level owing to lack a recombination system. Therefore, we developed gene system for M. using Agrobacterium tumefaciens-mediated transfer target FKB1, which encodes FKBP12 protein that binds calcineurin inhibitor tacrolimus. The wild-type strain was sensitive tacrolimus, whereas FKB1 deletion mutant resistant Reintroduction into restored levels susceptibility Moreover, an FKB1-eGFP fusion generated expression this observed cytoplasm. This newly will help further our understanding pathogenesis globosa-related level.